EC-Oxyrase®

"The enzyme Oxyrase specifically removes oxygen from the micro-environment but does not generate any other gas."

                                                      ~ Journal of Clinical Microbiology 

EC-Oxyrase® is a standardized, enzyme preparation available in two formats: sterile and non-sterile.

It is made to have a minimum of 30 Oxyrase Units per mL over its shelf life, if stored as specified.

The enzymatic activity of EC-Oxyrase® resides on membrane particles about 0.1 micron in size.

An Oxyrase Unit is defined as the amount of activity that reduces dissolved oxygen, at 37°C and pH 8.4 in a phosphate buffer with lactate as substrate, at the rate of 1% per second (see figure 1). Under these conditions, 0.3 unit of EC-Oxyrase® can reduce dissolved oxygen at saturation to levels measured in parts per billion (ppb) in about 4 minutes (see figure 2).

EC-Oxyrase is active over a wide temperature range (see figure 3). It can be kept at 40°C for hours without substantial loss of activity. It is heat inactivated above 55°C.

EC-Oxyrase® operates over a wide pH range of 6.8 to 9.4 (see figure 4). The optimum pH is about 8.0. As the pH moves away from optimum, activity decreases. The lower activity level can be compensated for by increasing time to complete oxygen removal or by increasing EC-Oxyrase® concentration.

EC-Oxyrase® requires substrate to be active. Levels at 10 mM to 20 mM are adequate.  See the Product Insert for additional information on substrates and their use.

To maintain stability, EC-Oxyrase® must be stored at constant ≤ -20°C. It may be thawed and refrozen several times without loss of activity.