Use OxyPlates™ for Subbing
The OxyPlate™ is changing the way today's clinical labs isolate and work with anaerobic pathogens. For microbiologists charged with growing anaerobes, OxyPlates™ bring a new level of convenience to a difficult job, and give microbiologists more control throughout the entire recovery process.
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| No need to change current methods. |
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OxyPlates™ are self-contained anaerobic chambers. They generate and maintain anaerobic environments .... again and again. The OxyPlate™ is incubated in a standard aerobic incubator. Each isolate can be handled individually and independently of all other isolates when using an OxyPlate. That isolate can be worked with when It Is Ready... not only when it is time to open the jar. OxyPlates frees you from the limitations of bags, jars, or anaerobic chambers. Think what this can do for work-flow and improved efficiency!
OxyPlates™ for subbing can be used independently of the means used to set primary plates. Think of OxyPlates™ "in addition to" rather than "instead of" your current method. OxyPlates™ complement all existing methodologies. |
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In evidence based microbiology, Best Practice incorporates the consensus state of the art developed by experts and published in peer review literature. Best Practice provides the most  reliable approach to obtaining a desired outcome and should be used by non-experts working in the field. Others, outside the field, would judge the propriety of the work done by comparing it to Best Practice.
To isolate and grow anaerobes, the literature teaches, you need:
1. a suitable, anaerobic environment , and
2. a suitable medium prepared for anaerobes.
The OxyPlate™ meets both of these criteria. The OxyPlate™ generates its own anaerobic environment. It is not dependent on an external device to create anaerobiosis. The OxyPlate™ can be opened and closed, and it regenerates an anaerobic environment again and again. The medium in an OxyPlate™ is made PRAS. Because of The Oxyrase® Enzyme System, the OxyPlate™ comes to you reduced and ready to use. Oxyrase® is a dynamic reducing agent that keeps the medium reduced when it is opened in air. The OxyPlate™ has Best Practice built in. There is nothing extra for you to do or to supply, when you use the OxyPlate™. Could isolating anaerobes be simpler?
If you don't use Best Practice for working with anaerobes, how can you be sure about what you really have – and what you may be missing?
To understand the importance of media preparation for isolating and growing anaerobes, read
' Why PRAS Media' in the box below ( references provided on request ).
Why PRAS Media?
Only PRAS media meet the requirements of all clinical anaerobes for isolation and growth. Anaerobe microorganisms are among the most difficult microbes to isolate and grow because they need an environment that's both free of oxygen and reduced. The reduced environment is physiologically necessary for anaerobes to produce energy for growth.
Absence of oxygen and a reduced environment are related but not identical. It is possible to have an environment devoid of oxygen, but not sufficiently reduced to grow some anaerobes. To meet this requirement, microbiologists add reducing agents (such as thioglycollate and cysteine) to media formulated to grow anaerobes. But when a medium with reducing agents is sterilized by autoclaving and the medium contains oxygen, the reducing agents are among the first molecules to be oxidized. This lessens the reduced state that can be achieved in the final medium.
In addition, the presence of organic peroxides formed during sterilization of the medium can inhibit anaerobe growth. Once formed during medium preparation, these oxidized products can not be removed by placing the cooled, finished medium into an anaerobic and reduced environment.
The only way to eliminate these oxidation products is to prevent them from being formed during media preparation. This requires that the medium is first made anaerobic before reducing agents are added and then sterilized in the reduced state. This has become known as PRAS media...Pre-Reduced – Anaerobically Sterilized media. |
Working with anaerobes is perhaps the most time consuming and costly activity in the clinical microbiology laboratory. However, media cost is but a small part of this total cost. Does it make sense to invest in the laboratory equipment and supplies for anaerobes, train microbiology technicians to do the work, and then skimp on media to ' save money ' ? When considering cost for subbing anaerobes, take into account savings from the OxyPlate™ replacing the bag, jar, or chamber and the accompanying accessories. In addition, the OxyPlate™ improves laboratory efficiency. It makes working with anaerobes simpler. Simple saves time and money. Simple is better!
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The Oxyrase® enzyme removes oxygen from the plated media environment naturally, selectively and efficiently. OxyPlates™ are capable of growing known clinical anaerobes.
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The Technology Behind Oxyrase®
...was discovered by the late Dr. Howard Adler and his colleagues at the biology division of Oak Ridge National Laboratory in Tennessee. Dr. Adler and his colleagues found that cell-membrane fractions – derived from Escherichia coli and related microorganisms – were specific for oxygen, reducing it directly to water and capable of creating an anaerobic environment when added to any liquid or solid culture media.
The result was Oxyrase® – a new method of making media for anaerobes and for working with microorganisms in an aerobic environment. |
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Compare OxyPlates™ to your current plates used to sub anaerobes ... you will notice the difference.
- OxyPlates™ are made for anaerobes using a biotech method that enhances the plates.
- They come reduced and ready to use ... there's nothing for you to prep.
- OxyPlates™are specially packaged in oxygen barrier bags to assure the anaerobic integrity of their environment during storage.
- They can be stored at room temperature.
- OxyPlates™ can be stored in a refrigerator, dramatically extending their shelf life.
- OxyPlates™ are cost effective when all cost factors are taken into account.
- OxyPlates™ generate anaerobic conditions again and again.
- Compare Customer Service .. Never Back Ordered .. We Ship Directly to You.
- OxyPlates™ have Best Practice Built In
- OxyPlates™ can be bought at:
Oxyrase, Inc.
Cardinal Healthcare
Fisher Scientific
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But don't take my word for it...
Test the performance of OxyPlates™ for subbing. We'll send you OxyPlates™ FREE...yours to test with no obligation. Click here to order your free test samples now. Or call 1-888-699-3733, ext. 225 to order your FREE test samples of OxyPlates™ immediately! |
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| "Oxyrase is an enzyme mixture coveted by microbiologists for its unique ability to remove O2 from media in which anaerobic bacteria are grown." |
| Journal of the American Society of Nephrology |
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I sincerely believe that, once you try OxyPlates™ for subbing anaerobe isolates, you'll discover for yourself how...
- OxyPlates™ enhance the growth and isolation of anaerobes.
- OxyPlates™ come ready to use and stay reduced even after extended exposure to atmospheric oxygen.
- OxyPlates™ eliminate hassles you've always associated with anaerobe recovery.
- OxyPlates™ simplify the anaerobe recovery process.
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So don't delay. Order your free test samples of OxyPlates™ now. Discover for yourself the unprecedented convenience and control you'll get with OxyPlates™ used for subbing.
2009 © Oxyrase, Inc. All Rights Reserved.
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